An Experimental assessment of the influence of Ganoderma Lucidum on the state of Oxidative stress

Abstract

This article describes the capabilities of Ganoderma Lucidum in the correction of oxidative stress. It has been proven that Ganoderma Lucidum removes free radicals, acts as antioxidants and enhances innate immunity. Materials and research methods: in this article data of histological, biochemical and ultramicroscopic research methods are given. Results of the study: according to the results of a histological study in the 3 main group, in which Ganoderma Lucidum was used, active regeneration of the liver was observed, as evidenced by the frequent mitosis of hepatocytes, fatty degeneration is less pronounced than in 1 and 2 main groups. in which olive oil and 5% -2.0 ml of ascorbic acid were used. In the 3 main group after exposure to Ganoderma Lutsidum, the proportion of intact hepatocytes was 68 ± 2.0% (p≤0.05) compared with 2 and 3 main groups (32.0 ± 3.5%, p≤0.05). The study showed that the activity of cytochrome c in 2 and 3 main groups was significantly higher than in 1 main group. In particular, this is due to the effect of triterpene fractions and β-δ-polyglucans to increase liver cytochrome oxidase due to an increase in the nonspecific resistance of the organism, as a result of which the optical density of the solution decreases. Moreover, in the 3rd main group, the activity of cytochrome oxidase was higher by 37.8% (p≤0.05) than in the 1st and 2nd main groups and amounted to 59.0 ± 1.05 nmol of succinate / mg protein per min (at a rate of 61 , 09 ± 1.09 nmol of succinate / mg protein per minute). In groups 1 and 2, the activity of cytochrome c was reduced by 38% (p≤0.01) and 44% (p≤0.01), respectively. As a result, after an increase in the activity of cytochrome c, endogenous redox reactions and metabolic processes in tissues accelerated, oxygen utilization improved, and hypoxia in tissues decreased during CCl4 poisoning.