THE ROLE OF IL6 GENE POLYMORPHISM –174C/G IN THE DEVELOPMENT OF RECURRENT BRONCHIAL OBSTRUCTION IN CHILDREN OF EARLY SCHOOL AGE

Abstract

The purpose of our work was to analyze the polymorphism of the IL6 gene –174C/G in the development of recurrent bronchitis in children of the Uzbek population of preschool age. Material and methods. To achieve this goal, 73 cases of the disease were analyzed, of which group 1 included children with acute obstructive bronchitis (AOB), group 2 - with recurrent bronchial obstruction (RBO) aged 7 to 11 years. Determination of IL-6 and total IgE in blood serum was carried out by enzyme-linked immunosorbent assay using a commercial kit of enzyme-linked immunosorbents. Total genomic DNA was isolated using the DNA-Express kit (Litekh). Single nucleotide polymorphism of the IL-6 gene 174G/C was determined by PCR. Statistical processing of the obtained data was carried out using Microsoft Excel 2013. Differences were considered statistically significant at p < 0.05. Results. A comparative analysis of IL6 and total IgE in AOB and RBO showed a significant increase in IL-6 and an increase in the level of IgE in RBO compared with the AOB group and healthy children. A study of the IL6 –174C/G gene polymorphism showed that the G/G mutacin genotype was observed significantly more often in the group of patients with RBO than in the healthy group. Conclusion: It follows that the main allele G is associated with an increased level of IL-6 expression and is a prognostic factor for the development of RBO in children. The obtained clinical, genetic and immunological results of the study allow us to predict RBR in children.