THE EFFECT OF NEW PLANT HEPATOPROTECTORS ON THE LEVEL OF PROINFLAMMATORY CYTOKINES IN ACUTE TOXIC LIVER DAMAGE

Abstract

Purpose of the research: a comparative study of the molecular mechanisms of the antiinflammatory action of catacin, geranium, cavergal in comparison with carlsil in a model of acute toxic liver damage with heliotrin. Material and methods. Studies were carried out on 118 adult male rats. The model of acute toxic damage (ATD) was reproduced by a single subcutaneous injection of heliotrin at a dose of 200 mg / kg of animal body weight in 110 rats, 8 rats made up the intact group. On the 3rd day of the toxicant administration, the surviving animals were divided into 5 groups: 1) ATD + physiological saline at a dose of 0.5 ml / 100 g body weight (control) of 20 rats; 2) ATD + carsil (comparison group) 19 rats; 3) ATD + catacin 19 rats; 4) ATD + geranyl of 19 rats; 5) ATD + cover 18 rats. The drugs were administered intragastrically daily in the morning at 100 mg / kg for 6 and 12 days. 24 hours after the final administration of the plant preparations, the animals were killed under mild anesthesia. The development of toxic hepatitis was judged by the activity of ALT and AST, the content of bilirubin and its fractions in serum using a MINDRAYBA-88A biochemical analyzer (China) using commercial reagent kits from CYPRESS Diagnostics (Belgium). The content of IL-1β, IL-6 and TNF-α was determined by enzyme-linked immunosorbent assay on an ELYZA apparatus (Germany) using Bender Medsystems kits. For statistical processing of the results, Exell and OriginPro7.5 software packages (OriginLab Corporation, USA) were used. Results. The results of the study showed a sharp increase in the level of pro-inflammatory cytokines in ATD rats, especially those responsible for the humoral immunity. Experimental pharmacotherapy with new drugs from the group of flavonoids and proanthacyanidins leads to a decrease in the production of proinflammatory cytokines, and catacin and geranyl preparations turned out to be more effective in this regard. Conclusion: the studied new herbal preparations catacin and geranyl in rats with ATD significantly suppressed the high level of pro-inflammatory cytokines, which may indicate their anti-inflammatory activity