Abstract:
Purpose of the research: a comparative study of the molecular mechanisms of the antiinflammatory action of catacin, geranium, cavergal in comparison with carlsil in a model of acute toxic liver
damage with heliotrin. Material and methods. Studies were carried out on 118 adult male rats. The model of acute
toxic damage (ATD) was reproduced by a single subcutaneous injection of heliotrin at a dose of 200 mg / kg of
animal body weight in 110 rats, 8 rats made up the intact group. On the 3rd day of the toxicant administration, the
surviving animals were divided into 5 groups: 1) ATD + physiological saline at a dose of 0.5 ml / 100 g body weight
(control) of 20 rats; 2) ATD + carsil (comparison group) 19 rats; 3) ATD + catacin 19 rats; 4) ATD + geranyl of 19
rats; 5) ATD + cover 18 rats. The drugs were administered intragastrically daily in the morning at 100 mg / kg for 6
and 12 days. 24 hours after the final administration of the plant preparations, the animals were killed under mild
anesthesia. The development of toxic hepatitis was judged by the activity of ALT and AST, the content of bilirubin
and its fractions in serum using a MINDRAYBA-88A biochemical analyzer (China) using commercial reagent kits
from CYPRESS Diagnostics (Belgium). The content of IL-1β, IL-6 and TNF-α was determined by enzyme-linked
immunosorbent assay on an ELYZA apparatus (Germany) using Bender Medsystems kits. For statistical processing
of the results, Exell and OriginPro7.5 software packages (OriginLab Corporation, USA) were used. Results. The
results of the study showed a sharp increase in the level of pro-inflammatory cytokines in ATD rats, especially those
responsible for the humoral immunity. Experimental pharmacotherapy with new drugs from the group of flavonoids
and proanthacyanidins leads to a decrease in the production of proinflammatory cytokines, and catacin and geranyl
preparations turned out to be more effective in this regard. Conclusion: the studied new herbal preparations catacin
and geranyl in rats with ATD significantly suppressed the high level of pro-inflammatory cytokines, which may
indicate their anti-inflammatory activity
