Рerspectives for optimizing the molecular genetic laboratory in early diagnosis and prediction of multifactorial diseases in women and children

Abstract

The widespread use of molecular genetic methods in multifactorial diseases makes it possible to improve the prediction of pathology long before the onset of clinical signs, which significantly helps in the prevention of the disease. Based on the study of molecular genetics and functional char­acteristics of children with acute obstructive pulmonary disease (AOB) and recurrent bronchial obstruction (RBO) with a predisposition to bronchial asthma (BA), the optimization of early diagnosis and medical rehabilitation measures was carried out. The distribution frequencies of alleles and gen­ otypes of the Arg16Gly (rs1042713) and Gln27Glu (rs1042714) locus of the β2-adrenergic receptor (ADRB2) locus, children with RBO, OOB, AD, and healthy children were studied by real-time PCR using the SNP-express-SHOT reagent kit. on modern equipment "Rotor Gene 6000/Q". The need for specialized molecular genetic laboratories with certified equipment based on multidisciplinary clin­ics is a promising direction in optimizing the early diagnosis of diseases in children.